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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two additional splice variants ended up documented but detailed nucleotide sequence facts just isn't however out there.

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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.

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This tutorial outlines even further achievable optimization of cellular and immunoassay parameters to be certain the very best benefits are...

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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody as opposed to the antibody alone, you can see which staining is specific.

Target Data Cyclin E belongs to your hugely conserved cyclin family members, whose customers are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which add for the temporal coordination of each mitotic celebration. Cyclin E sorts a fancy with and functions as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary which is degraded as cells development by means of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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Clicking the photographs or hyperlinks will redirect you to an internet site hosted by BenchSci that gives 3rd-occasion scientific information. Neither the content nor the BenchSci technologies and processes for range happen to be evaluated by us; click here we're delivering them as-is and devoid of guarantee of any sort, together with to be used or application on the Thermo Fisher Scientific solutions offered.